Secretion of recombinant proteins fromE. coli
نویسندگان
چکیده
منابع مشابه
Recombinant protein secretion in Escherichia coli.
The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of soluble and biologically active proteins at a red...
متن کاملProduction of recombinant proteins in Escherichia coli.
E. coli is the expression system of choice and a substantial body of literature has accumulated on the successful expression of foreign genes in this host. Several problems with protein expression in E. coli have been encountered, and many have been ultimately solved. This unit describes methods that have been developed for production of recombinant proteins in E. coli and potential pitfalls th...
متن کاملMaximized Autotransporter-Mediated Expression (MATE) for Surface Display and Secretion of Recombinant Proteins in Escherichia coli.
A new optimized system for the surface display and secretion of recombinant proteins is described, termed MATE (maximized autotransporter-mediated expression). It is based on an artificial gene consisting of the coding region for the signal peptide of CtxB, a multiple cloning site for passenger gene insertion, flanked by coding sequences for linear epitopes for monoclonal antibodies and OmpT, a...
متن کاملSecretion of recombinant proteins into the culture medium by Escherichia coli and Staphylococcus aureus
Many human proteins with the potential to be used as therapeutics are secreted. These include growth factors, cytokines and protein hormones. The eukaryotic secretion process often involves a range of post-translational modifications which are required for correct tertiary structure and which have direct implications for biological activity as well as serum half-life. These modifications can in...
متن کاملSecretion of recombinant proteins into the culture medium by Escherichia coli and Staphylococcus aureus.
The ability of staphylococcal protein A (SPA) to bind to the Fc part of IgG has been used for the purification of a number of heterologous gene products as fusion proteins. Both the SPA promoter and signal sequence function in Escherichia coli, as well as in a number of Gram-positive bacteria, which facilitates comparisons of the expressed specific products in different hosts. The expression sy...
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ژورنال
عنوان ژورنال: Engineering in Life Sciences
سال: 2018
ISSN: 1618-0240
DOI: 10.1002/elsc.201700200